J Periodontal Implant Sci.
2021 Oct;51(5):329-341. 10.5051/jpis.2102600130.
Identification of stemness and differentially expressed genes in human cementum-derived cells
- Affiliations
-
- 1Dental Research Institute, Seoul National University, Seoul, Korea
- 2Department of Periodontics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea
- 3Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea
- 4Department of Dentistry, Uijeongbu Eulji Medical Center, Eulji University, Uijeongbu, Korea
- KMID: 2557511
- DOI: http://doi.org/10.5051/jpis.2102600130
Abstract
- Purpose
Periodontal treatment aims at complete regeneration of the periodontium, and developing strategies for periodontal regeneration requires a deep understanding of the tissues composing the periodontium. In the present study, the stemness characteristics and gene expression profiles of cementum-derived cells (CDCs) were investigated and compared with previously established human stem cells. Candidate marker proteins for CDCs were also explored.
Methods
Periodontal ligament stem cells (PDLSCs), pulp stem cells (PULPSCs), and CDCs were isolated and cultured from extracted human mandibular third molars. Human bone marrow stem cells (BMSCs) were used as a positive control. To identify the stemness of CDCs, cell differentiation (osteogenic, adipogenic, and chondrogenic) and surface antigens were evaluated through flow cytometry. The expression of cementum protein 1 (CEMP1) and cementum attachment protein (CAP) was investigated to explore marker proteins for CDCs through reverse-transcription polymerase chain reaction. To compare the gene expression profiles of the 4 cell types, mRNA and miRNA microarray analysis of 10 samples of BMSCs (n=1), PDLSCs (n=3), PULPSCs (n=3), and CDCs (n=3) were performed.
Results
The expression of mesenchymal stem cell markers with a concomitant absence of hematopoietic markers was observed in PDLSCs, PULPSCs, CDCs and BMSCs. All 4 cell populations also showed differentiation into osteogenic, adipogenic, and chondrogenic lineages. CEMP1 was strongly expressed in CDCs, while it was weakly detected in the other 3 cell populations. Meanwhile, CAP was not found in any of the 4 cell populations. The mRNA and miRNA microarray analysis showed that 14 mRNA genes and 4 miRNA genes were differentially expressed in CDCs vs. PDLSCs and PULPSCs.
Conclusions
Within the limitations of the study, CDCs seem to have stemness and preferentially express CEMP1. Moreover, there were several up- or down-regulated genes in CDCs vs. PDLSCs, PULPSCs, and BMSCs and these genes could be candidate marker proteins of CDCs.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Identification of differentially expressed genes using an annealing control primer system in periodontitis
- Idenfitied Differentially Expressed Genes in Keratoconus
- Identification of CCL1 as a Gene Differentially Expressed in CD4+ T Cells Expressing TIM-3
- Identification of Up-Regulated Genes in Malignant Glioma with Subtraction Hybridization: Preliminary Screening Studies
- Isolation and Characterization of Human Suture Mesenchymal Stem Cells In Vitro
