J Periodontal Implant Sci.  2023 Dec;53(6):417-428. 10.5051/jpis.2301920096.

Effect of polydeoxyribonucleotide with xenogeneic collagen matrix on gingival phenotype modification: a pilot preclinical study

• Lim HC
• Kim CH
• Lee Hk
• Jeon G
• Herr Y
• Chung JH

• KMID: 2557418
• DOI: http://doi.org/10.5051/jpis.2301920096

Abstract

Purpose
To investigate the effect of xenogeneic collagen matrix (XCM) with polydeoxyribonucleotide (PDRN) for gingival phenotype modification compared to autogenous connective tissue graft.
Methods
Five mongrel dogs were used in this study. Box-type gingival defects were surgically created bilaterally on the maxillary canines 8 weeks before gingival augmentation. A coronally positioned flap was performed with either a subepithelial connective tissue graft (SCTG) or XCM with PDRN (2.0 mg/mL). The animals were sacrificed after 12 weeks. Intraoral scanning was performed for soft tissue analysis, and histologic and histomorphometric analyses were performed.
Results
One animal exhibited wound dehiscence, leaving 4 for analysis. Superimposition of STL files revealed no significant difference in the amount of gingival thickness increase (ranging from 0.69±0.25 mm to 0.80±0.31 mm in group SCTG and from 0.48±0.25 mm to 0.85±0.44 mm in group PDRN; P>0.05). Histomorphometric analysis showed no significant differences between the groups in supracrestal gingival tissue height, keratinized tissue height, tissue thickness, and rete peg density P>0.05).
Conclusions
XCM soaked with PDRN yielded comparable gingival augmentation to SCTG.

Keyword

Alveolar ridge augmentation; Animal model; Autografts; Polydeoxyribonucleotide; Tissue scaffolds