J Nutr Health.
2024 Feb;57(1):43-52. 10.4163/jnh.2024.57.1.43.
The anti-cancer effect of pomegranate-derived nanovesicles on MDA-MB-231 breast cancer cells
- Affiliations
-
- 1Department of Molecular Medicine, Cell & Matrix Research Institute, Kyungpook National University, Daegu 41944, Republic of Korea
- 2Department of Food and Nutrition, Andong National University, Andong 36729, Republic of Korea
- KMID: 2553704
- DOI: http://doi.org/10.4163/jnh.2024.57.1.43
Abstract
- Purpose
Cancer is the leading cause of death in Koreans, with breast cancer being the most common among women. Breast cancer readily metastasizes, and the existing treatment processes impose a significant burden on patients. This study examined whether pomegranate-derived exosome-like nanovesicles (PNVs) have anti-cancer effects by inhibiting cell infiltration and metastasis while increasing apoptosis on breast cancer MDA-MB-231 cells.
Methods
Initially, exosome-like nanovesicles were isolated from pomegranate using ultracentrifugation. Subsequently, the size range of these nanovesicles was confirmed using nanoparticle tracking analysis. The ability of breast cancer MDA-MB-231 cells to internalize these natural nanovesicles was assessed with flourescence microscope. The anti-cancer effects of the PNVs were confirmed by applying various concentrations of PNVs (10, 50, 100 μg/mL) to MDA-MB-231 cells and systematically assessing their impact on cell viability and migration.
Results
The round shape of the lipid bilayer in the PNVs was confirmed, providing crucial insights into their structural properties. We demonstrate that PNVs-associated DiD dye can be efficiently internalized by the MDA-MB-231 cells. The data showed that the PNVs inhibited cell viability, invasion rates, and migration in MDA-MB-231 cells. In addition, PNVs were absorbed into the MDA-MB-231 cells, leading to an increased expression of apoptosis proteins, such as cleaved caspase-3 and phosphorus-JNK, in a concentration-dependent manner. Furthermore, a reduction in cell infiltration and decreased expression of the transition markers MMP-2 and MMP-9 proteins were observed.
Conclusion
For the first time, this study suggests that PNVs may be useful in the prevention or treatment of breast cancer by inhibiting the infiltration and metastasis of MDA-MB-231 cells and inducing apoptosis.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of Curcumin on Cancer Invasion and Matrix Metalloproteinase-9 Activity in MDA-MB-231 Human Breast Cancer Cell
- Comparative Studies on the Polyarnine Involvement in MCF - 7 and MDA - MB - 231 Breast Cancer Cell Proliferation
- Ellagic Acid Shows Different Anti-proliferative Effects Between the MDA-MB-231 and MCF-7 Human Breast Cancer Cell Lines
- Effect of corosolic acid on apoptosis and angiogenesis in MDA-MB-231 human breast cancer cells
- Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells
