Lab Anim Res.  2024 Mar;40(1):51-63. 10.1186/s42826-024-00190-x.

Morin ameliorates myocardial injury in diabetic rats via modulation of inflammatory pathways

•  Verma V
•  Malik S
• Mutneja E
• Sahu A
• Prajapati V
• Mishra P
• Bhatia J
• Arya D

• KMID: 2553469
• DOI: http://doi.org/10.1186/s42826-024-00190-x

Abstract

Background
High blood glucose levels in diabetes lead to vascular inflammation which accelerates atherosclerosis. Herein, Morin was orally administered in male Wistar rats, at the dose of 40 mg/kg for 28 days, and on the 27th and 28th day, ISO was administered to designate groups at the dose of 85 mg/kg s.c., to induce myocardial infarction.
Results
Free radical generation, including ROS, in diabetes following ISO administration, leads to the activation of both intrinsic and extrinsic pathways of apoptosis. Morin significantly (p ≤ 0.05) reduced oxidative stress (GSH, MDA, SOD), cardiac injury markers (CK-MB, LDH), inflammation (TNF, IL-6), and apoptosis (Bax, ­BCl ₂ , Caspase-3). In addition, it also reduced insulin and blood glucose levels. Akt/eNOS, Nrf2/HO-1, MAPK signaling pathways, and Insulin signal transduction pathways were positively modulated by morin pre-treatment.
Conclusions
Morin attenuated oxidative stress and inflammation and also modified the activity of various molecular pathways to mitigate cardiomyocyte damage during ISO-induced MI in diabetic rats.

Keyword

Isoproterenol; Diabetes; Myocardial necrosis; Molecular signaling pathway