Ann Lab Med.  2024 Mar;44(2):179-182. 10.3343/alm.2023.0252.

Concurrent Pleural and Pericardial Involvement in a Patient With De Novo Pure Erythroid Leukemia

• Zhang Y
• Li K
• Li X
• Wang H
• Li T
• Long F

• KMID: 2553390
• DOI: http://doi.org/10.3343/alm.2023.0252

Figure

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  Fig. 1 Results of peripheral blood and bone marrow aspirate analysis. (A) Peripheral blood smears show 9% large circulating blasts with deeply basophilic cytoplasm and large violet cytoplasmic inclusions (Wright–Giemsa stain, ×1000). (B) Bone marrow aspirate exhibits markedly increased “blasts” (constituting 81%) of intermediate to large size, a high nuclear-to-cytoplasmic ratio, round nuclei, dispersed chromatin, one to several prominent nucleoli and agranular basophilic cytoplasm, with occasional multiple purple inclusions and microvacuoles on some blasts (Wright–Giemsa stain, ×1000). (C) Periodic acid-Schiff staining reveals prominent globular inclusions (×1000). (D) Cytogenetics revealing a complex karyotype. (E) Bone marrow biopsy showing a sheet of blasts with fine chromatin and prominent nucleoli (hematoxylin–eosin stain, ×100). (F) Immunohistochemical staining positive for E-cadherin (×400).

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  Fig. 2 Results of bone marrow immunophenotyping analysis for (A) CD34/CD38, (B) CD117/HLA-DR, (C) CD71/CD235a, (D) CD71/CD33, (E) CD45/CD13, and (F) CD105/CD36 combinations. Flow-cytometric analysis for immunophenotyping showed blasts expressing CD13, CD33, CD36, CD71, CD105, and CD117, with partial expression of CD34, CD38, and human leukocyte antigen DR isotype, suggesting erythroid differentiation.

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