Parasit Host Dis.  2024 Feb;62(1):75-84. 10.3347/PHD.23108.

In vitro immunoregulatory role of recombinant Ancylostoma ceylanicum calreticulin

• Zhuang T
• Abuzeid AMI
• Chen X
• Zhu S
• Li G

• KMID: 2552999
• DOI: http://doi.org/10.3347/PHD.23108

Abstract

Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host’s immune response. However, no data on A. ceylanicum calreticulin (Ace-CRT) are available. We investigated the biological function of recombinant Ace-CRT (rAce-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that rAce-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. rAce-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. Our findings indicate that Ace-CRT plays an immunomodulatory role and may be a promising candidate molecule for a hookworm vaccine.

Keyword

recombinant calreticulin; cytokine expression