Korean J Parasitol.
2013 Dec;51(6):747-749. 10.3347/kjp.2013.51.6.747.
Molecular Detection of Ancylostoma duodenale, Ancylostoma ceylanicum, and Necator americanus in Humans in Northeastern and Southern Thailand
- Affiliations
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- 1Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. pewpan@kku.ac.th
- 2Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
- 3Department of Medicine, Mahasarakham University, Mahasarakham 44150, Thailand.
- 4Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
- 5Department of Parasitology, Faculty of Medicine Chiang Mai University, Chiang Mai 50200, Thailand.
- KMID: 1712232
- DOI: http://doi.org/10.3347/kjp.2013.51.6.747
Abstract
- The 2 principal species of hookworms infecting humans are Necator americanus and Ancylostoma duodenale. Case studies on zoonotic hookworm infections with Ancylostoma ceylanicum and/or Ancylostoma caninum are known mainly from Asian countries. Of these 2 zoonotic species, only A. ceylanicum can develop to adulthood in humans. In the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern Thailand. Thirty larval hookworm samples were obtained from fecal agar plate cultures of 10 patients in northeastren Thailand and 20 in southern Thailand. Partial ITS1, 5.8S, and ITS2 regions of the ribosomal DNA genes were amplified using PCR. The amplicons were sequenced, aligned, and compared with other hookworm sequences in GenBank database. The results showed that, in Thailand, N. americanus is more prevalent than Ancylostoma spp. and is found in both study areas. Sporadic cases of A. ceylanicum and A. duodenale infection were seen in northeastern Thailand.
Keyword
MeSH Terms
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Ancylostoma/classification/genetics/*isolation & purification
Ancylostomiasis/*epidemiology
Animals
Cluster Analysis
DNA, Ribosomal Spacer/chemistry/genetics
Feces/parasitology
Humans
Molecular Sequence Data
Necator americanus/classification/genetics/*isolation & purification
Necatoriasis/*epidemiology
Phylogeny
Polymerase Chain Reaction
Prevalence
RNA, Ribosomal, 5.8S/genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Thailand/epidemiology
DNA, Ribosomal Spacer
RNA, Ribosomal, 5.8S
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