Oral Biol Res.  2023 Dec;47(4):132-139. 10.21851/obr.47.04.202312.132.

Apoptotic effects of hyperoside on FaDu human pharyngeal carcinoma cells

• Kang KR
• Seo JY
• Lim H
• Kim JS
• Kim DK

• KMID: 2550104
• DOI: http://doi.org/10.21851/obr.47.04.202312.132

Abstract

Hyperoside (quercetin 3-o-β-d-galactopyranoside) is a flavonoid glycoside with antidepressant, anti-inflammatory, and anti-cancer effects. However, it is not known whether it is effective for oral cancer. This study aimed to investigate the effects of hyperoside on induction of apoptosis in FaDu human pharyngeal carcinoma cells. It employed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, live/dead staining, 4′,6-diamidino-2-phenylindole (DAPI) staining, hematoxylin and eosin staining, and western blotting to analyze FaDu cells. The MTT assay, live/dead staining, and DAPI staining showed that hyperoside increased FaDu cell apoptosis in a concentration-dependent manner. Hyperoside-induced apoptosis in FaDu cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Western blotting results showed that Bcl-2 and Bcl-xL were downregulated but Bad and Bax were upregulated by hyperoside in FaDu cells. These results suggest that hyperoside inhibits cell proliferation in FaDu human pharyngeal carcinoma cells and induces apoptosis through receptor-mediated extrinsic apoptosis and mitochondrial-mediated endogenous apoptosis pathways.

Keyword

Apoptosis; FaDu; Hyperoside; Oral cancer