Immune Netw.
2022 Jun;22(3):e27. 10.4110/in.2022.22.e27.
Characterization of Immune Cells From the Lungs of Patients With Chronic Non-Tuberculous Mycobacteria or Pseudomonas aeruginosa Infection
- Schenkel AR
1 - Mitchell JD2
- Cool CD3,4
- Bai X5,6
- Groshong S3,4
- Koelsch T7
- Verma D1
- Ordway D1
- Chan ED5,6,8,9
- Affiliations
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- 1Department of Microbiology, Immunology, & Pathology, Colorado State University, Fort Collins, CO 80523, USA
- 2Division of Cardiothoracic Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
- 3Department of Pathology, National Jewish Health, Denver, CO 80206, USA
- 4Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
- 5Department of Academic Affairs, National Jewish Health, Denver, CO 80206, USA
- 6Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
- 7Department of Radiology, National Jewish Health, Denver, CO 80206, USA
- 8Department of Medicine, National Jewish Health, Denver, CO 80206, USA
- 9Pulmonary Section, Rocky Mountain Regional Veterans Affairs Medical Center, Aurora, CO 80045, USA
- KMID: 2536254
- DOI: http://doi.org/10.4110/in.2022.22.e27
Abstract
- Little is known of the lung cellular immunophenotypes in patients with non-tuberculous mycobacterial lung disease (NTM-LD). Flow-cytometric analyses for the major myeloid and lymphoid cell subsets were performed in less- and more-diseased areas of surgically resected lungs from six patients with NTM-LD and two with Pseudomonas aeruginosa lung disease (PsA-LD). Lymphocytes, comprised mainly of NK cells, CD4 + and CD8 + T cells, and B cells, accounted for ~60% of all leukocytes, with greater prevalence of T and B cells in morediseased areas. In contrast, fewer neutrophils were found with decreased number in morediseased areas. Compared to NTM-LD, lung tissues from patients with PsA-LD demonstrated relatively lower numbers of T and B lymphocytes but similar numbers of NK cells. While this study demonstrated a large influx of lymphocytes into the lungs of patients with chronic NTM-LD, further analyses of their phenotypes are necessary to determine the significance of these findings.
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