J Korean Acad Oral Health.  2022 Jun;46(2):70-77. 10.11149/jkaoh.2022.46.2.70.

Lysozyme hydrochloride 0.01%, sodium fluoride 0.02%, cetylpyridinium chloride 0.05% antibacterial and sterilizing effect of mouth freshener

• Park HW
• Cho JW
• Yoo HJ
• Jeon YM
• Shin KH
• Ha SM

• KMID: 2530815
• DOI: http://doi.org/10.11149/jkaoh.2022.46.2.70

Abstract

Objectives
This study aimed to assess the antibacterial, bactericidal, and mouth freshener effects of lysozyme hydrochloride 0.01%, sodium fluoride 0.02%, and cetylpyridinium chloride 0.05%.
Methods
Eight oral disease-related bacteria were cultivated anaerobically. Four samples were prepared with or without 0.5% cetylpyridinium chloride, 0.2% sodium fluoride, and 0.1% lysozyme hydrochloride. Antimicrobial activity was tested in 96-well microplates. After assessing the bacterial count, the bacterial suspension was mixed with samples and spread on agar. The bactericidal rate was calculated by counting and comparing treated and untreated colonies.
Results
Lysozyme hydrochloride 0.01%, sodium fluoride 0.02%, and cetylpyridinium chloride 0.05% mouth fresheners sterilized 99.99% of 8 oral bacteria, including Streprococcus mutans. Lysozyme hydrochloride 0.01%, sodium fluoride 0.02%, and cetylpyridinium chloride 0.05% mouth fresheners showed 99.97% bactericidal activity against Lactobacillus acidophilus.
Conclusions
Lysozyme hydrochloride 0.01%, sodium fluoride 0.02%, and cetylpyridinium chloride 0.05% mouth fresheners confirmed the sterilization and antibacterial effects on oral disease-causing bacteria.

Keyword

Anti-bacterial agents; Cetylpyridinium chloride; Fluorides; Mouthwashes; Sterilization

Figure

• 

  Fig. 1 Antibacterial test results for S. mutans. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm. (*) indicates a statistically significant difference between experimental group 1 and experimental group 2 and experimental group 3 and experimental group 4 (P<0.05).

• 

  Fig. 2 Antibacterial test results for S. sobrinus. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm. (*) indicates a statistically significant difference between experimental group 1 and experimental group 2 and experimental group 3 and experimental group 4 (P<0.05).

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  Fig. 3 Antibacterial test results for L. acidophilus. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm. (*) indicates a statistically significant difference between experimental group 1 and experimental group 2 and experimental group 3 and experimental group 4 (P<0.05).

• 

  Fig. 4 Antibacterial test results for E. faecalis. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm.

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  Fig. 5 Antibacterial test results for A. israelii. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm.

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  Fig. 6 Antibacterial test results for A. actinomycetemcomitans. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm.

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  Fig. 7 Antibacterial test results for P. intermedia. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm.

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  Fig. 8 Antibacterial test results for P. nigrescens. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm.

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  Fig. 9 Antibacterial test results for P. gingivalis. Bacterial growth was measured by inoculating bacteria in a medium in which the solution of each experimental group was serially diluted 2 times using a medium and measuring absorbance at a wavelength of 660 nm.

Reference

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