J Wound Manag Res.  2022 Feb;18(1):11-16. 10.22467/jwmr.2021.01823.

Effects of Particulate Matter on Wound Healing: An In Vivo Study

• Lee HM
• Kim CS
• Na YC
• Yoon CS
• Kim JH
• Yun SS
• Park JH

• KMID: 2527213
• DOI: http://doi.org/10.22467/jwmr.2021.01823

Abstract

Background
Particulate matter (PM) is known to increase the risk of diseases in major organs and PM may also slow the complex process of wound healing. We investigated the effect of PM on wound healing in vivo.
Methods
Twelve 10-week-old Sprague-Dawley male rats were punctured with three 8 mm-diameter holes each in the skin on their backs. The holes were divided into three groups according to treatment: PM 50 µg/mL (group A), PM 100 µg/mL (group B) and saline (control). For histologic analysis, three rats were sacrificed on each of the 1st, 3rd, 7th, and 28th days, and a total of 36 samples were collected. Inflammatory cell infiltration and neovascularization were evaluated for comparison among groups.
Results
Inflammation increased in groups A and B on day 1, but no significance was observed. On the 28th day, increased inflammation was observed in groups A and B compared to the control group, with significant difference. Angiogenesis increased in groups A and B compared to the control group on day 1, but no significance was observed. On the 3rd day, there was a decrease in group B, with statistical significance. On day 28, it was observed that angiogenesis significantly increased in group B compared to the control group.
Conclusion
Inflammation increased in the PM groups compared with the control group and angiogenesis decreased, then slowly increased in the PM groups compared with the control group. Our findings suggest that PM increases inflammation and delays angiogenesis in the wound healing process.

Keyword

Particulate matter; Inflammation; Angiogenesis; Wound healing