J Korean Assoc Oral Maxillofac Surg.  2022 Feb;48(1):3-12. 10.5125/jkaoms.2022.48.1.3.

Next generation sequencing-based salivary biomarkers in oral squamous cell carcinoma

• Sodnom-Ish B
• Eo MY
• Myoung H
• Lee JH
• Kim SM

• KMID: 2526805
• DOI: http://doi.org/10.5125/jkaoms.2022.48.1.3

Abstract

Selection of potential disease-specific biomarkers from saliva or epithelial tissues through next generation sequencing (NGS)-based protein studies has recently become possible. The early diagnosis of oral squamous cell carcinoma (OSCC) has been difficult, if not impossible, until now due to the lack of an effective OSCC biomarker and efficient molecular validation method. The aim of this study was to summarize the advances in the application of NGS in cancer research and to propose potential proteomic and genomic saliva biomarkers for NGS-based study in OSCC screening and diagnosis programs. We have reviewed four categories including definitions and use of NGS, salivary biomarkers and OSCC, current biomarkers using the NGSbased technique, and potential salivary biomarker candidates in OSCC using NGS

Keyword

Next generation sequencing; Saliva; Biomarkers; Early diagnosis; Oral squamous cell carcinoma

Figure

• 

  Fig. 1 Schematic drawing of the Maxam and Gilbert’s chemical chain termination method for DNA sequencing developed in 1977 followed by Sanger’s ‘dideoxy method’9,10. (PCR: polymerase chain reaction)

Reference

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