.  2021 Mar;25(1):10-21. 10.32542/implantology.2021002.

Bone Regeneration Using Collagenated Synthetic Bone Graft Attached to Collagen Membrane with 3,4-dihydroxy-L-phenylalanine (DOPA)

• Hong I
• Dolgorsuren R
• Jeon SH
• Song YW
• Cha JK
• Lee JS
• Paik JW
• Choi SH

• KMID: 2521281
• DOI: http://doi.org/10.32542/implantology.2021002

Abstract

Purpose
The stability of graft material and membrane is crucial for successful guided bone regeneration. As 3,4-dihydroxy-L-phenylalanine (DOPA) is known to contribute to the adhesiveness of natural mussel adhesive proteins, the aim of this study was to evaluate the biocompatibility and bone regeneration using collagenated synthetic bone graft attached to the collagen membrane with DOPA in the rabbit calvaria model.
Materials and Methods
Four circular bone defects of 8 mm diameter were created in the rabbit calvarium. Each defect in the calvarium was randomly allocated to 1) sham control group, 2) bone graft using a particle-type synthetic bone substitute (OP) group, 3) bone graft using collagenated synthetic bone block (OC) group, or 4) bone graft using the collagenated synthetic bone graft attached to collagen membrane with DOPA (OC-DOPA) group. A total of 10 rabbits were sacrifced at either 2 or 8 weeks post-surgery.
Results
During histometric analysis, new bone formation in the OC-DOPA group was signifcantly greater than that in sham control at 8 weeks. On the contrary, there was no statistically signifcant difference in new bone area (NBA) between OC-DOPA and OC. Histologic analysis revealed infltrations of infammatory cells in the OP, OC, and OC-DOPA groups at 2 weeks. At 8 weeks, there were no remarkable infammatory cells in the OP and OC groups. However, multinucleated giant cells (MNGC) were observed till 8 weeks in the OC-DOPA group.
Conclusion
The collagenated synthetic bone graft attached to the collagen membrane using DOPA showed its potential for guided bone regeneration. However, MNGC were observed until the late healing period.