Tissue Eng Regen Med.  2021 Oct;18(5):905-913. 10.1007/s13770-021-00371-y.

The Combination of Concentrated Growth Factor and AdiposeDerived Stem Cell Sheet Repairs Skull Defects in Rats

• Hu T
• Zhang H
• Yu W
• Yu X
• Li Z
• He L

• KMID: 2520648
• DOI: http://doi.org/10.1007/s13770-021-00371-y

Abstract

BACKGROUND
The goal of this study was to create a biomaterial which combines concentrated growth factor (CGF) with an adipose-derived stem cell (ADSC) sheet to promote the repair of skull defects in rats.
METHODS
We determined the optimal concentration of CGF extract by investigating the effects of different concentrations (0, 5%, 10%, and 20%) on the proliferation and differentiation of ADSCs. Then we created a complex combining CGF with an ADSC sheet, and tested the effects on bone repair in four experimental rat groups: (A) control; (B) ADSC sheet; (C) CGF particles; (D) combination of CGF ? ADSCs. Eight weeks after the procedure, osteogenesis was assessed by micro-CT and hematoxylin and eosin staining.
RESULTS
We found that the concentration of CGF extract that promoted optimal ADSC proliferation and differentiation in vitro was 20%. In turn, bone regeneration was promoted the most by the combination of CGF and ADSCs.
CONCLUSION
In this study, we determined the optimal ratio of CGF and ADSCs to be used in a biomaterial for bone regeneration. The resulting CGF/ADSCs complex promotes maxillofacial bone defect repair in rats.

Keyword

Adipose-derived stem cells; Concentrated growth factor; Bone regeneration; Cell sheet