Lab Anim Res.  2021 Mar;37(1):90-97.

Comparison of age‐dependent alterations in thioredoxin 2 and thioredoxin reductase 2 expressions in hippocampi between mice and rats

  • 1Department of Emergency Medicine, Institute of Medical Sciences, School of Medicine, Kangwon National University Hospital, Kangwon National University, 24289, Chuncheon, Gangwon, Republic of Korea
  • 2Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangnung-Wonju National University, 25457, Gangneung, Gangwon, Republic of Korea
  • 3Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, 325035, Wenzhou, Zhejiang, P.R. China
  • 4Department of Neurobiology, School of Medicine, Kangwon National University, 24341, Chuncheon, Gangwon, Republic of Korea
  • 5Department of Physical Therapy, College of Health Science, Youngsan University, 50510, Yangsan, Gyeongnam, Republic of Korea
  • 6Department of Food Science and Nutrition, Hallym University, 24252, Chuncheon, Gangwon, Republic of Korea
  • 7Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, 24252, Chuncheon, Gangwon, Republic of Korea


Aging is one of major causes triggering neurophysiological changes in many brain substructures, including the hippocampus, which has a major role in learning and memory. Thioredoxin (Trx) is a class of small redox proteins. Among the Trx family, Trx2 plays an important role in the regulation of mitochondrial membrane potential and is controlled by TrxR2. Hitherto, age-dependent alterations in Trx2 and TrxR2 in aged hippocampi have been poorly investigated. Therefore, the aim of this study was to examine changes in Trx2 and TrxR2 in mouse and rat hippocampi by age and to compare their differences between mice and rats.
Trx2 and TrxR2 levels using Western blots in mice were the highest at young age and gradually reduced with time, showing that no significant differences in the levels were found between the two subfields. In rats, however, their expression levels were the lowest at young age and gradually increased with time. Nevertheless, there were no differences in cellular distribution and morphology in their hippocampi when it was observed by cresyl violet staining. In addition, both Trx2 and TrxR2 immunoreactivities in the CA1-3 fields were mainly shown in pyramidal cells (principal cells), showing that their immunoreactivities were altered like changes in their protein levels.
Our current findings suggest that Trx2 and TrxR2 expressions in the brain may be different according to brain regions, age and species. Therefore, further studies are needed to examine the reasons of the differences of Trx2 and TrxR2 expressions in the hippocampus between mice and rats.


Aging; Hippocampus; Rodent; Trx2; TrxR2
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