Lab Anim Res.
2021 Mar;37(1):44-52. 10.1186/s42826-020-00079-5.
Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources
- Park JW1
- Lee SJ1
- Kim JE1
- Kang MJ1
- Bae SJ1
- Choi YJ1
- Gong JE1
- Kim KS2
- Jung YS3
- Cho JY4
- Choi YS5
- Hwang DY
1 - Song HK6
- Affiliations
-
- 1Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animals Resources Center, Pusan National University, Miryang, South Korea
- 2College of Veterinary Medicine, Kyungpook National University, Daegu, South Korea
- 3College of Pharmacy, Pusan National University, Busan, South Korea
- 4Exercise Biochemistry Laboratory, Korea National Sport University, Seoul, South Korea
- 5Department of Biomedical Analysis, Korea Bio Polytechnic College, Nonsan, South Korea
- 6Central Research Institute, Kinesiences Co., Seoul, South Korea
- KMID: 2514374
- DOI: http://doi.org/10.1186/s42826-020-00079-5
Abstract
- Sepsis, one of the most fatal diseases in the world, is known to culminate in multiple organ failure due to an uncontrolled inflammatory response. Hence, the use of animal models in sepsis research is very important to study complex immune responses. The current study was undertaken to compare commercial stocks with KFDA stocks of DBA/2 mice as an animal model for sepsis study. To compare responses of DBA/2 mice to lipopolysaccharides (LPS)-induced sepsis, we measured altered characteristics of various factors associated with sepsis, including survival curves, organ failure and inflammatory response, in DBA/2Korl stock and two commercial stocks (DBA/2A and DBA/ 2B). Survival rates after LPS exposure were similar for DBA/2Korl and DBA/2B; however, for times over 20 h, survival rates were reduced and concentration dependent in DBA/2A. In order to evaluate multiple organ failure caused by sepsis, H&E stains were evaluated for liver and spleen tissues obtained in the early (2 h) and later (20 h) stages after exposure to LPS; no significant differences were observed between the three stocks. mRNA and protein levels of proinflammatory cytokines were assessed for evaluating inflammatory reactions, and were found to increase in a dose-dependent manner in most DBA/2 mice after LPS treatment. However, no changes were observed in the mRNA levels of proinflammatory cytokines at 20 h after LPS exposure in the DBA/2A stock. The induction of inflammation-mediated factors by LPS exposure did not induce alterations in the mRNA levels of COX-2 and iNOS in all three DBA/2 stocks. Our results indicate that response of DBA/2Korl to LPS-induced sepsis is similar to the two commercial DBA/2 stocks, thus representing its potential as a useful biological resource established in Korea.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of amnion derived stem cells on inflammatory response in endotoxemic rats
- Comparison of therapeutic responses to an anticancer drug in three stocks of ICR mice derived from three different sources
- A comparative study of the phenotype with kainic acid-induced seizure in DBA/2 mice from three different sources
- MIR210HG Aggravates Sepsis-Induced Inflammatory Response of Proximal Tubular Epithelial Cell via the NF-κB Signaling Pathway
- Effect of D-glucose feeding on mortality induced by sepsis
