Lab Anim Res.  2020 Dec;36(4):303-309. 10.1186/s42826-020-00072-y.

A comparative study of the phenotype with kainic acid-induced seizure in DBA/2 mice from three different sources

Affiliations
  • 1Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Republic of Korea
  • 2KPC Corporation, Gwangju 12773, Korea.
  • 3College of Pharmacy, Pusan National University, Busan 46241, Korea.
  • 4Exercise Biochemistry Laboratory, Korea National Sport University, 88-15 Oryung-dong, Songpa-gu, Seoul 138-763, Korea
  • 5Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea.
  • 6Department of Histology, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea
  • 7College of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, Korea.

Abstract

The kainic acid-induced seizure mouse model is widely used in epilepsy research. In this study, we applied kainic acid to the subcutaneous injections of three different sources of DBA/2 mice to compare and evaluate the seizure response. The three mouse sources consisted of DBA/2Kor1 (Korea FDA source), DBA/2A (USA source), and DBA/2 (Japan source), and were purchased from different vendors. To compare the responses of DBA/2 mice to kainic acid injections, we examined the survival rate, seizure phenotype scoring, and behavioral changes. We also evaluated brain lesions using histopathological analysis. Following the administration of kainic acid, almost half of the cohort survived, and the seizure phenotype displayed a moderate level of sensitivity (2 ~ 4 out of 6). In the histopathologic analysis, there was no change in morphological features, and levels of glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba-1) increased in the kainic acid-treated groups. However, there was no difference in the neuronal nuclei (NeuN) expression level. All the data showed that the responses in the kainic acid-treated group were similar across the three strains. In conclusion, our results suggest that the three sources of DBA/2 mice (DBA/2Kor1, DBA/2A, and DBA/2B) have similar pathological responses to kainic acid-induced seizures.

Keyword

DBA/2Kor1; Kainic acid; Seizure; Brain; DBA/2 mouse
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