Clin Exp Reprod Med.  2020 Sep;47(3):180-185. 10.5653/cerm.2020.03573.

Localization (and profiles) of tyrosinephosphorylated proteins in female reproductive organs of adult rats

• Bunsueb S
• Tangsrisakda N
• Wu AT
• Iamsaard S

• KMID: 2506142
• DOI: http://doi.org/10.5653/cerm.2020.03573

Abstract

Objective
Tyrosine phosphorylation is an essential process in many biological systems, including the male reproductive system. The presence of tyrosine-phosphorylated (TyrPho) proteins has been well documented in male reproductive organs, but research in fertile females is still limited.
Methods
The ovary, oviduct, and uterus of adult female Sprague-Dawley rats in the estrus phase were used to localize TyrPho proteins using an immunohistochemical technique. These proteins were separated and their expression patterns were examined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and Western blot analysis, respectively.
Results
TyrPho proteins were localized in the cytoplasm of the oocyte except the antral fluid; in the granulosa cells, theca cells, and stromal cells of the ovary; at the apical surface of oviductal epithelial cells; and in the basal epithelium and submucosa of the uterine wall. Moreover, we found that 72-, 43-, and 28-kDa TyrPho proteins were localized in the ovary, while 170-, 55-, and 43-kDa proteins were localized in the oviduct. In the uterus, we detected four major bands, corresponding to 61-, 55-, 54-, and 43-kDa TyrPho proteins.
Conclusion
Given that these TyrPho proteins were found in major reproductive organs in the estrus phase, these proteins may play important roles in female fertility.

Keyword

Ovary; Oviduct; Phosphorylation; Rats; Uterus