Lab Anim Res.  2019 Aug;35(2):70-77. 10.1186/s42826-019-0010-4.

Comparison study of the response with botulinum toxin muscle injection in the ICR mice from three different sources

  • 1Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Korea.
  • 2KPC Coporation, Gwangju 12773, Korea.
  • 3Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, Korea.
  • 4Department of Health and Exercise Science, Korea National Sport University, 88-15 Oryung-dong, Songpa-gu, Seoul, Korea.
  • 5Central Research Institute, Kine sciences, F1, Milovany, 28, Inchon-ro, Seongbuk-gu, Seoul, Korea.
  • 6Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.
  • 7Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.
  • 8College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.


Botulinum-toxin A (BoNT/A) is a widely used not only for cosmetics but also for various experimental purposes including muscle-related research. In this study, we applied BoNT/A to mouse muscle of three different sources to compare and evaluate the biological and pathological response. The three different mouse sources consist of Korl: ICR (Korea FDA source), A:ICR (USA source) and B:ICR (Japan source) which were purchased from each different vendors. To compare the responses of ICR mice with BoNT/A muscle injection, we examined the body weight, hematological and serum biochemistry analysis. Also, we evaluated the muscle change by histopathological analysis and gene expression patterns of muscle-related target by qPCR. The body weight gain was decreased in the BoNT/ A-treated group compared with the control group. In clinical pathologic analysis and gene expression patterns, the data showed that the responses in the BoNT/A-treated group were similar compared with the control group. Decreased muscle fiber was observed in BoNT/A-treated group compared with control group, while Korl:ICR showed a little low response with the other mouse sources. In conclusion, our results suggest that three different sources ICR mice (Korl:ICR, A:ICR and B:ICR) have a similar biological and pathological responses in BoNT/A muscle injection.


Korl:ICR; Botulinum toxin; Muscle; ICR mouse

MeSH Terms

Body Weight
Botulinum Toxins*
Gene Expression
Mice, Inbred ICR*
Botulinum Toxins
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