Int J Stem Cells.  2019 Jul;12(2):360-366. 10.15283/ijsc18125.

MBP-FGF2-Immobilized Matrix Maintains Self-Renewal and Myogenic Differentiation Potential of Skeletal Muscle Stem Cells

Affiliations
  • 1Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University, Cheonan, Korea. jkyoon@sch.ac.kr, yshwang0428@sch.ac.kr
  • 2Department of Integrated Biomedical Science, Graduate School, Soon Chun Hyang University, Asan, Korea.
  • 3Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
  • 4Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea. skimbrc@kist.re.kr

Abstract

The robust capacity of skeletal muscle stem cells (SkMSCs, or satellite cells) to regenerate into new muscles in vivo has offered promising therapeutic options for the treatment of degenerative muscle diseases. However, the practical use of SkMSCs to treat muscle diseases is limited, owing to their inability to expand in vitro under defined cultivation conditions without loss of engraftment efficiency. To develop an optimal cultivation condition for SkMSCs, we investigated the behavior of SkMSCs on synthetic maltose-binding protein (MBP)-fibroblast growth factor 2 (FGF2)-immobilized matrix in vitro. We found that the chemically well-defined, xeno-free MBP-FGF2-immobilized matrix effectively supports SkMSC growth without reducing their differentiation potential in vitro. Our data highlights the possible application of the MBP-FGF2 matrix for SkMSC expansion in vitro.

Keyword

Skeletal muscle stem cell; Satellite cell; MBP-FGF2; Self-renewal; Myogenic differentiation

MeSH Terms

In Vitro Techniques
Maltose-Binding Proteins
Muscle, Skeletal*
Muscles
Stem Cells*
Maltose-Binding Proteins
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