Int J Stem Cells.  2019 Nov;12(3):430-439. 10.15283/ijsc19067.

Reprogramming of Cancer Cells into Induced Pluripotent Stem Cells Questioned

Affiliations
  • 1Department of Stem Cell Biology, Konkuk University School of Medicine, Seoul, Korea. knko@kku.ac.kr
  • 2Center for Stem Cell Research, Institute of Advanced Biomedical Science, Konkuk University, Seoul, Korea.
  • 3Department of Medicine, College of Medicine, Chung-Ang University, Seoul, Korea.
  • 4Research Institute of Medical Science, Konkuk University, Seoul, Korea.

Abstract

BACKGROUND AND OBJECTIVES
Several recent studies have claimed that cancer cells can be reprogrammed into induced pluripotent stem cells (iPSCs). However, in most cases, cancer cells seem to be resistant to cellular reprogramming. Furthermore, the underlying mechanisms of limited reprogramming in cancer cells are largely unknown. Here, we identified the candidate barrier genes and their target genes at the early stage of reprogramming for investigating cancer reprogramming.
METHODS
We tried induction of pluripotency in normal human fibroblasts (BJ) and both human benign (MCF10A) and malignant (MCF7) breast cancer cell lines using a classical retroviral reprogramming method. We conducted RNA-sequencing analysis to compare the transcriptome of the three cell lines at early stage of reprogramming.
RESULTS
We could generate iPSCs from BJ, whereas we were unable to obtain iPSCs from cancer cell lines. To address the underlying mechanism of limited reprogramming in cancer cells, we identified 29 the candidate barrier genes based on RNA-sequencing data. In addition, we found 40 their target genes using Cytoscape software.
CONCLUSIONS
Our data suggest that these genes might one of the roadblock for cancer cell reprogramming. Furthermore, we provide new insights into application of iPSCs technology in cancer cell field for therapeutic purposes.

Keyword

Induced pluripotent stem cells; iPSC generation; RNA-sequencing analysis; Pluripotency; Cancer cell reprogramming

MeSH Terms

Breast Neoplasms
Cell Line
Cellular Reprogramming
Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Methods
Transcriptome
Zidovudine
Zidovudine
Full Text Links
  • IJSC
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr