J Periodontal Implant Sci.  2018 Aug;48(4):224-235. 10.5051/jpis.2018.48.4.224.

Effects of platelet-rich plasma on tooth replantation in dogs: a histologic and histomorphometric analysis

• Yang JM
• Yang KI
• Lee KH
• Choi SH
• Kim BO
• Park JC
• Yu SJ

• KMID: 2465388
• DOI: http://doi.org/10.5051/jpis.2018.48.4.224

Abstract

PURPOSE
The purpose of this study was to evaluate the effects of platelet-rich plasma (PRP) on periodontal healing of replanted root surfaces in dogs histologically and histomorphometrically.
METHODS
A total of 36 roots of mandibular incisors and premolars from 6 mongrel dogs were used. The roots were randomly divided into 3 groups: 1) a positive control group (n=12), in which the periodontal ligament (PDL) and cementum were retained and the roots were soaked in saline; 2) a negative control group (n=12), in which the PDL and cementum were removed and the roots were soaked in saline; and 3) an experimental group (n=12), in which the PDL and cementum were removed and the roots were soaked in PRP. After soaking the root surfaces, the extracted roots were replanted into the extraction sockets. The roots were covered using a coronally repositioned flap
RESULTS
Histologically, irregular-thickness PDL-like and cementum-like tissues were observed in the 4-week experimental group and the positive control group. PDL-like tissue and cementum-like tissue with a more uniform thickness were observed at 8 weeks. In the negative control group, PDL-like tissue and cementum-like tissue were rarely found, and root resorption and ankylosis were observed. In the cross-sectional histomorphometric analysis, the experimental group demonstrated a higher rate of formation of cementum-like tissue and a lower tooth ankylosis rate than the positive and negative control groups at 4 and 8 weeks. Although there was a significant difference in the tooth ankylosis rate and the formation of cementum-like tissue across the 3 groups (P < 0.05), no statistical significance was observed between any pair of groups (P > 0.017).
CONCLUSIONS
Applying PRP to root surfaces during tooth replantation in dogs can reduce tooth ankylosis and increase PDL-like and cementum-like tissue formation.

Keyword

Dental cementum; Periodontal ligament; Periodontitis; Platelet-rich plasma; Tooth replantation

MeSH Terms

Animals
Ankylosis
Bicuspid
Clothing
Dental Cementum
Dogs*
Incisor
Periodontal Ligament
Periodontitis
Platelet-Rich Plasma*
Root Resorption
Tooth Ankylosis
Tooth Replantation*
Tooth*