Korean J Orthod.  2019 Nov;49(6):372-380. 10.4041/kjod.2019.49.6.372.

The influence of leukocyte-platelet-rich plasma on accelerated orthodontic tooth movement in rabbits

• Nakornnoi
• Leethanakul C
• Samruajbenjakun B

• KMID: 2464200
• DOI: http://doi.org/10.4041/kjod.2019.49.6.372

Abstract

OBJECTIVE
To determine the effects of a local injection of leukocyte-platelet-rich plasma (L-PRP) on orthodontic tooth movement in rabbits.
METHODS
Twenty-three male New Zealand white rabbits were included in a split-mouth design. Tooth movement with a 100-g nickel-titanium closed-coil spring was performed on the maxillary first premolars. L-PRP was injected submucosally at the buccal and lingual areas of the first premolar in one random side of the maxilla and the other side served as the control and received normal saline. The amount of tooth movement was assessed on three-dimensional digital models on days 0, 3, 7, 14, 21, and 28. Histological findings and osteoclast numbers were examined on day 0 as the baseline and on days 7, 14, and 28.
RESULTS
The L-PRP group showed significantly greater cumulative tooth movement at all observed periods. However, a significantly higher rate of tooth movement was observed only on days 0-7 and 7-14. The osteoclast numbers were significantly increased in the L-PRP group on days 7 and 14.
CONCLUSIONS
Local injection of L-PRP resulted in a transient increase in the rate of tooth movement and higher osteoclast numbers.

Keyword

Leukocyte-platelet-rich plasma; Orthodontics; Tooth movement

MeSH Terms

Bicuspid
Humans
Male
Maxilla
Orthodontics
Osteoclasts
Plasma*
Rabbits*
Tooth Movement*
Tooth*

Figure

• 

  Figure 1 Schematic of the study design and timeline. L-PRP, Leukocyte-platelet-rich plasma; NSS, normal saline solution.

• 

  Figure 2 (A) Platelet and (B) leukocyte concentrations in the whole blood and leukocyte-platelet-rich plasma (L-PRP).

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  Figure 3 A, Cumulative distance of tooth movement in the leukocyte-platelet-rich plasma (L-PRP) and control groups. B, Rate of tooth movement in the L-PRP and control groups. *p < 0.05; **p < 0.01; ***p = 0.001.

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  Figure 4 The osteoclast numbers in the leukocyte-platelet-rich plasma (L-PRP) and control groups. *p < 0.05.

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  Figure 5 Histological sections showing the compression side of the maxillary first premolar in the leukocyte-platelet-rich plasma (L-PRP) (A, C, E, G) and control groups (B, D, F, H) on days 0, 7, 14 and 28, respectively (H&E; ×20). The inflammatory cells (black arrows) and osteoclasts (arrowheads) were magnified in the images (C, E) at the bottom right corner (40-times magnification). PDL, Periodontal ligament; Alv, alveolar bone.

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