J Biomed Transl Res.  2019 Sep;20(3):58-64. 10.12729/jbtr.2019.20.3.058.

Ventilation accelerates lung injury in septic mice

  • 1Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.
  • 2Department of Pediatrics, Chonnam National University Children's Hospital and Medical School, Gwangju 61469, Korea.
  • 3Department of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital, Gwangju 61469, Korea.
  • 4College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea.
  • 5College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea. yudh@gnu.ac.kr


Sepsis is one of the systemic inflammatory responses, and it can cause acute respiratory distress syndrome (ARDS). Mechanical ventilation (MV) is one of the best treatments in patients with sepsis related to ARDS. However, mechanical ventilation itself can cause lung injury through various mechanisms. In this study, we applied mouse cecal ligation and puncture (CLP) model of sepsis with subsequent injurious ventilation to determine if sepsis could affect the development of ventilator-induced lung injury (VILI). To evaluate lung injury caused by sepsis, mild, moderate, and severe CLPs were induced. To evaluate lung injury caused by MV with or without sepsis, tidal volume (7 mL/kg, 10 mL/kg, or 24 mL/kg) was maintained for 3 h with or without septic insult (mild sepsis for 72 h). Lung compliance was then determined and biochemical evaluations were performed. Results showed that lung compliance was lower in the severe sepsis group compared to that in the mild or moderate sepsis group. MV alone decreased lung compliance in a tidal volume dependent manner. Both CLP and VILI groups showed significant decrease in lung compliance and increase in total cell count upon bronchoalveolar lavage fluid. These results confirm that ventilation can affect lung injury in mouse sepsis model.


sepsis; acute respiratory distress syndrome; ventilator-induced lung injury; cecal ligation and puncture; animal model

MeSH Terms

Bronchoalveolar Lavage Fluid
Cell Count
Lung Compliance
Lung Injury*
Models, Animal
Respiration, Artificial
Respiratory Distress Syndrome, Adult
Tidal Volume
Ventilator-Induced Lung Injury
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