Korean J Parasitol.  2019 Aug;57(4):435-437. 10.3347/kjp.2019.57.4.435.

Detection of Human Anti-Trypanosoma cruzi Antibody with Recombinant Fragmented Ribosomal P Protein

• Kim YH
• Yang Z
• Lee J
• Ahn HJ
• Chong CK
• Maricondi
• Dias RF
• Nam HW

• KMID: 2459483
• DOI: http://doi.org/10.3347/kjp.2019.57.4.435

Abstract

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity against positive sera of patients, and we cloned the protein after fragmenting it to enhance its antigenicity and solubility. Twelve positive sera of Chagas disease patients were reacted with the fragmented ribosomal P protein using western blot. Detection rate and density for each fragment were determined. Fragments F1R1, F1R2, and F2R1 showed 100% rate of detection, and average density scoring of 2.00, 1.67, and 2.42 from a maximum of 3.0, respectively. Therefore, the F2R1 fragment of the ribosomal P protein of T. cruzi could be a promising antigen to use in the diagnosis of Chagas disease in endemic regions with high specificity and sensitivity.

Keyword

Trypanosoma cruzi; Chagas disease; ribosomal P protein; GST-fusion protein; western blot; patients' sera

MeSH Terms

Blotting, Western
Chagas Disease
Clone Cells
Diagnosis
Humans*
Parasites
Sensitivity and Specificity
Serologic Tests
Solubility
Trypanosoma cruzi