Korean J Parasitol.
2015 Aug;53(4):483-488. 10.3347/kjp.2015.53.4.483.
Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms
- Affiliations
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- 1Departamento de Microbiologia, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, Londrina, PR, Brazil. lionilmy@uel.br
- 2Departamento de Microbiologia, Imunobiologia e Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil.
- 3Departamento de Ciencias Patologicas, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, Londrina, PR, Brazil.
- 4Conselho de Pos-graduacao e Pesquisa, Universidade Bandeirante de Sao Paulo, Sao Paulo, SP, Brazil.
- 5Departamento de Ciencias Basicas da Saude, Centro de Ciencias da Saude, Universidade Estadual de Maringa, Maringa, PR, Brazil.
- 6Departamento de Parasitologia, Microbiologia e Imunologia, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil.
- KMID: 2381767
- DOI: http://doi.org/10.3347/kjp.2015.53.4.483
Abstract
- This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.
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