Lab Med Online.  2019 Oct;9(4):254-257. 10.3343/lmo.2019.9.4.254.

A Case of Anti-Le(bH) Antibody Identified in a Patient with Ulcerative Colitis

• Hong J
• Kim MS
• Kim JS
• Park H
• Ko DH
• Hwang SH
• Oh HB

• KMID: 2458688
• DOI: http://doi.org/10.3343/lmo.2019.9.4.254

Abstract

A 67-year-old man previously diagnosed with ulcerative colitis complained of difficulty in defecation and underwent balloon dilatation of rectum, but the procedure failed. The patient was transferred to a surgical department for further treatment. Before surgery, his red cells were typed A, Rh(D) positive. The antibody screening test was positive and the results of the identification tests were atypical. The reactivity was similar to anti-Le(b) antibody; however, the antibody showed panreactivity against papainized red cells. It showed stronger reactivity against O red cells than A Le(a−b+) red cells, and we concluded that the antibody was anti-Le(bH). After reexamination, his Lewis phenotype was found to be Le(a−b−). His FUT2 and FUT3 were analyzed to confirm his Lewis blood type, and c.59T>G and c.1067T>A variants were found on the FUT3. Therefore, the patient's Lewis blood type was concluded as Le(a−b−).

Keyword

Lewis blood group; Anti-Le(bH); Ulcerative colitis; FUT3

MeSH Terms

Aged
Colitis, Ulcerative*
Defecation
Dilatation
Humans
Mass Screening
Papain
Phenotype
Rectum
Ulcer*
Papain

Figure

• 

  Fig. 1 Sequencing chromatogram of the FUT3. In coding exons, c.59T>G (p.Leu20Arg) variant (A) and c.1067T>A (p.Ile356Lys) variant (B) were found in homozygous states. Red arrows indicate the locations of the variants.

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