Clin Exp Reprod Med.  2019 Sep;46(3):107-111. 10.5653/cerm.2018.00423.

Investigation of the association of idiopathic male infertility with polymorphisms in the methionine synthase (MTR) gene

• Tanoomand
• Hajibemani A
• Abouhamzeh B

• KMID: 2458497
• DOI: http://doi.org/10.5653/cerm.2018.00423

Abstract

OBJECTIVE
Spermatogenesis is a complex process that is regulated by a number of genes, some of which are involved in folate-dependent 1-carbon metabolism. Methionine synthase (encoded by MTR) is a key enzyme participating in this pathway. This study aimed to investigate the relationship of the MTR 2756A > G polymorphism with idiopathic male fertility in the Iranian population.
METHODS
The participants of this study included 100 men with idiopathic infertility and 100 healthy men as the control group. Genotyping of MTR 2756A > G was performed using the polymerase chain reaction and restriction fragment length polymorphism technique. The obtained data were analyzed using SPSS ver. 20.0 with a level of confidence of p< 0.05.
RESULTS
The frequencies of the A and G alleles at this locus were 77% and 23% in infertile patients and 84% and 16% in the control group, respectively. The frequencies of the GG, GA, and AA genotypes were 5%, 36%, and 59% in the infertile patients versus 3%, 27%, and 70% in the control group, respectively. No significant difference was observed in any genetic models.
CONCLUSION
In general, the findings of this study suggest that the MTR 2756A > G single-nucleotide polymorphism is not a predisposing factor for idiopathic infertility in men.

Keyword

Idiopathic; Male infertility; MTR; Polymorphism

MeSH Terms

5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase*
Alleles
Causality
Fertility
Genotype
Humans
Infertility
Infertility, Male*
Male
Male*
Metabolism
Methionine*
Models, Genetic
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Spermatogenesis
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Methionine