Lab Anim Res.  2019 Jun;35(1):23-29. 10.1186/s42826-019-0002-4.

Comparison of the virulence of Streptococcus pneumoniae in ICR mouse stocks of three different origins

• Kim JY
• Seo SM
• Lee HK
• Kim HW
• Choi YK

• KMID: 2456057
• DOI: http://doi.org/10.1186/s42826-019-0002-4

Abstract

Streptococcus pneumoniae causes many people to suffer from pneumonia, septicemia, and other diseases worldwide. To identify the difference in susceptibility of and treatment efficacy against S. pneumoniae in three ICR mouse stocks (Korl: ICR, A:ICR, and B:ICR) with different origins, mice were infected with 2 × 106, 2×107, and 2×108 CFU of S. pneumoniae D39 intratracheally. The survival of mice was observed until three weeks after the infection. The three stocks of mice showed no significant survival rate difference at 2 × 106 and 2 × 107 CFU. However, the lung and spleen weight in the A:ICR stock was significantly different from that in the other two stocks, whereas the liver weight in B:ICR stock was significantly lower than that in the other two stocks. Interestingly, no significant CFU difference in the organs was observed between the ICR stocks. The level of interferon gamma inducible protein 10 in Korl:ICR was significantly lower than that in the other two stocks. The level of granulocyte colony stimulating factor in B:ICR was significantly lower than in the other two stocks. However, tumor-necrosis factor-alpha and interleukin-6 levels showed no significant difference between the ICR stocks. In the vancomycin efficacy test after the S. pneumoniae infection, both the single-dose and double-dose vancomycin-treated groups showed a significantly better survival rate than the control group. There was no significant survival difference between the three stocks. These data showed that Korl:ICR, A:ICR, and B:ICR have no susceptibility difference to the S. pneumoniae D39 serotype 2.

Keyword

Streptococcus pneumonia D39; Vancomycin; Survival rate

MeSH Terms

Animals
Colony-Stimulating Factors
Granulocytes
Interferons
Interleukin-6
Liver
Lung
Mice
Mice, Inbred ICR*
Pneumonia
Sepsis
Serogroup
Spleen
Streptococcus pneumoniae*
Streptococcus*
Survival Rate
Treatment Outcome
Vancomycin
Virulence*
Colony-Stimulating Factors
Interferons
Interleukin-6
Vancomycin