Mycobiology.  2019 Jun;47(2):217-229. 10.1080/12298093.2019.1589900.

Two Manganese Peroxidases and a Laccase of Trametes polyzona KU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

• Lueangjaroenkit P
• Teerapatsakul C
• Sakka K
• Sakka M
• Kimura T
• Kunitake E
• Chitradon

• KMID: 2455702
• DOI: http://doi.org/10.1080/12298093.2019.1589900

Abstract

Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from Trametes polyzona KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25"‰mg/L) in 10-30"‰min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were 50"‰°C and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.

Keyword

Manganese peroxidase; Trametes polyzona; dye decolorization; pharmaceutical product degradation; redox mediator-free system

MeSH Terms

Amoxicillin
Biodegradation, Environmental
Ciprofloxacin
Doxycycline
Hydrogen-Ion Concentration
Ions
Laccase*
Manganese*
Oxidation-Reduction*
Peroxidases*
Pharmaceutical Preparations
Solvents
Tetracycline
Trametes*
Amoxicillin
Ciprofloxacin
Doxycycline
Ions
Laccase
Manganese
Peroxidases
Pharmaceutical Preparations
Solvents
Tetracycline