Mycobiology.
2012 Dec;40(4):258-262. 10.5941/MYCO.2012.40.4.258.
Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
- Affiliations
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- 1Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. smpark@chonbuk.ac.kr
- 2Institute of Sunchang Fermented Soybean Products, Sunchang 595-805, Korea.
- KMID: 1428540
- DOI: http://doi.org/10.5941/MYCO.2012.40.4.258
Abstract
- cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 microM within 90 min.
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