Mycobiology.  2012 Dec;40(4):258-262. 10.5941/MYCO.2012.40.4.258.

Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

Affiliations
  • 1Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. smpark@chonbuk.ac.kr
  • 2Institute of Sunchang Fermented Soybean Products, Sunchang 595-805, Korea.

Abstract

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 microM within 90 min.

Keyword

Dye decolorization; Glyoxal oxidase; Manganese peroxidase; Phanerochaete chrysosporium; Pichia pastoris

MeSH Terms

Alcohol Oxidoreductases
DNA, Complementary
Glyoxal
Manganese
Organometallic Compounds
Oxidoreductases
Peroxidase
Peroxidases
Phanerochaete
Pichia
Rosaniline Dyes
Alcohol Oxidoreductases
DNA, Complementary
Glyoxal
Manganese
Organometallic Compounds
Oxidoreductases
Peroxidase
Peroxidases
Rosaniline Dyes
Full Text Links
  • MB
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error