Korean J Pain.  2019 Jul;32(3):168-177. 10.3344/kjp.2019.32.3.168.

Diverse characters of Brennan’s paw incision model regarding certain parameters in the rat

Affiliations
  • 1Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India. raysb48@gmail.com

Abstract

BACKGROUND
Brennan's rodent paw incision model has been extensively used for understanding mechanisms underlying postoperative pain in humans. However, alterations of physiological parameters like blood pressure and heart rate, or even feeding and drinking patterns after the incision have not been documented as yet. Moreover, though eicosanoids like prostaglandins and leukotrienes contribute to inflammation, tissue levels of these inflammatory mediators have never been studied. This work further investigates the antinociceptive effect of protein C after intra-wound administration.
METHODS
Separate groups of Sprague-Dawley rats were used for quantitation of cyclooxygenase (COX) activity and leukotriene B4 level by enzyme-linked immunosorbent assay, as well as estimation of cardiovascular parameters and feeding and drinking behavior after paw incision. In the next part, rats were subjected to incision and 10 μg of protein C was locally administered by a micropipette. Both evoked and non-evoked pain parameters were then estimated.
RESULTS
COX, particularly COX-2 activity and leukotriene B4 levels increased after incision. Hemodynamic parameters were normal. Feeding and drinking were affected on days 1 and 3, and on day 1, respectively. Protein C attenuated non-evoked pain behavior alone up to day 2.
CONCLUSIONS
Based upon current observations, Brennan's rodent paw incision model appears to exhibit a prolonged period of nociception similar to that after surgery, with minimal interference of physiological parameters. Protein C, which is likely converted to activated protein C in the wound, attenuated the guarding score, which probably represents pain at rest after surgery in humans.

Keyword

Blood Pressure; Eicosanoids; Heart Rate; Inflammation; Nociception; Pain, Postoperative; Protein C; Rats; Wounds and Injuries

MeSH Terms

Animals
Blood Pressure
Drinking
Drinking Behavior
Eicosanoids
Enzyme-Linked Immunosorbent Assay
Heart Rate
Hemodynamics
Humans
Inflammation
Leukotriene B4
Leukotrienes
Nociception
Pain, Postoperative
Prostaglandin-Endoperoxide Synthases
Prostaglandins
Protein C
Rats*
Rodentia
Wounds and Injuries
Eicosanoids
Leukotriene B4
Leukotrienes
Prostaglandin-Endoperoxide Synthases
Prostaglandins
Protein C
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