Yonsei Med J.  2012 Sep;53(5):1036-1044.

Gene Expression Pattern of Human Chorion-Derived Mesenchymal Stem Cells during Adipogenic Differentiation

• Kim YH
• Park TC
• Lee G
• Shin JC

Abstract

PURPOSE
The aim of this study was to identify the adipocyte-specific gene expression patterns in chorion-derived mesenchymal stem cells during adipogenic differentiation.
MATERIALS AND METHODS
Chorionic cells were isolated from the third trimester chorions from human placenta at birth and identified morphologically and by fluorescence-activated cell sorting analysis. After inducing adipogenic differentiation for 28 days, cells at days 3, 10, 21 and 28 were analyzed by Oil red O staining and RNA extraction in order to assess the expression levels of adipocyte marker genes, including CCAAT-enhancer binding protein alpha (C/EBPalpha), peroxisome proliferator-activated receptor gamma (PPARgamma), fatty acid binding protein 4 (FABP4) and Glycerol-3-phosphate dehydrogenase (GPD2). Cells not induced for differentiation were compared with the induced cells as a control group.
RESULTS
Chorion-derived cells showed the same pattern as fibroblasts, and expressed CD73, CD105, and CD166 antigens, but not CD45, CD34, and HLA-DR antigens. On day 3 after differentiation, cells began to stain positively upon Oil red O staining, and continuously increased in lipid granules for 4 weeks. The expression level of C/EBPalpha increased 4.6 fold on day 3 after induction, and continued to increase for 4 weeks. PPARgamma was expressed at a maximum of 2.9 fold on day 21. FABP4 and GPD2 were significantly expressed at 4.7- and 3.0-fold, respectively, on day 21, compared to controls, and further increased thereafter.
CONCLUSION
Human chorion-derived mesenchymal stem cells exhibited the sequential expression pattern of adipocyte marker genes during differentiation, corresponding to adipogenesis.

Keyword

Chorion; mesenchymal stem cells; adipogenesis; differentiation; gene expression

MeSH Terms

Activated-Leukocyte Cell Adhesion Molecule
Adipocytes
Adipogenesis
Carrier Proteins
Chorion
Female
Fibroblasts
Flow Cytometry
Gene Expression*
Glycerolphosphate Dehydrogenase
HLA-DR Antigens
Humans*
Mesenchymal Stromal Cells*
Parturition
Placenta
PPAR gamma
Pregnancy
Pregnancy Trimester, Third
RNA
Activated-Leukocyte Cell Adhesion Molecule
Carrier Proteins
Glycerolphosphate Dehydrogenase
HLA-DR Antigens
PPAR gamma
RNA