J Vet Sci.  2019 Jul;20(4):e33. 10.4142/jvs.2019.20.e33.

Antibacterial effects of sodium tripolyphosphate against Porphyromonas species associated with periodontitis of companion animals

Affiliations
  • 1Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul 02447, Korea. ljinyong@khu.ac.kr, prudence75@khu.ac.kr
  • 2Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Korea.
  • 3Department of Preventive Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, Korea.
  • 4Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea.
  • 5Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea.

Abstract

Porphyromonas species are closely associated with companion animal periodontitis which is one of the most common diseases in dogs and cats and leads to serious systemic diseases if left untreated. In this study, we evaluated the antimicrobial effects and mode of action of sodium tripolyphosphate (polyP3, Na5P3O10), a food additive with proven safety, using three pathogenic Porphyromonas species. The minimum inhibitory concentrations (MICs) of polyP3 against Porphyromonas gulae, Porphyromonas cansulci, and Porphyromonas cangingivalis were between 500 and 750 mg/L. PolyP3 significantly decreased viable planktonic cells as well as bacterial biofilm formation, even at sub-MIC concentrations. PolyP3 caused bacterial membrane disruption and this effect was most prominent in P. cangingivalis, which was demonstrated by measuring the amount of nucleotide leakage from the cells. To further investigate the mode of action of polyP3, high-throughput whole-transcriptome sequencing was performed using P. gulae. Approximately 30% of the total genes of P. gulae were differentially expressed by polyP3 (> 4-fold, adjusted p value < 0.01). PolyP3 influenced the expression of the P. gulae genes related to the biosynthesis of thiamine, ubiquinone, and peptidoglycan. Collectively, polyP3 has excellent antibacterial effects against pathogenic Porphyromonas species and can be a promising agent to control oral pathogenic bacteria in companion animals.

Keyword

Porphyromonas; tripolyphosphate; antibacterial; companion animals; periodontitis

MeSH Terms

Animals
Bacteria
Biofilms
Cats
Dogs
Food Additives
Friends*
Humans
Membranes
Microbial Sensitivity Tests
Peptidoglycan
Periodontitis*
Pets*
Plankton
Porphyromonas*
Sodium*
Thiamine
Ubiquinone
Food Additives
Peptidoglycan
Sodium
Thiamine
Ubiquinone
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