J Biomed Transl Res.
2019 Jun;20(2):44-52. 10.12729/jbtr.2019.20.2.044.
Novel porcine model of acute myocardial infarction using polyethylene terephthalate
- Kim YS
- Kim NY
- Bae IH
- Park JK
- Park DS
- Shim JW
- Kang WS
- Kim HB
- Song BS
- Koo BS
- Jeong KJ
- Kim SU
- Kim HK
- Kim SS
- Kim MC
- Sim DS
- Hong YJ
- Ahn Y
- Lim KS
- Jeong MH
- Affiliations
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- 1Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare, Gwangju 61469, Korea. myungho@chollian.net, mhjeong@chonnam.ac.kr
- 2Cardiovascular Research Center, Chonnam National University Hospital, Gwangju 61469, Korea.
- 3Departments of Thoracic and Cardiovascular Surgery and Cardiology, Wonkwang University School of Medicine, Iksan 54538, Korea.
- 4Korea Cardiovascular Stent Research Institute, Jangsung 57248, Korea.
- 5CGBio Co. Ltd, Jangseong 57248, Korea.
- 6Research Institute of Medical Sciences, Chonnam National University, Gwangju 61186, Korea.
- 7Futuristic Animal Resource and Research Center & National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 28116, Korea. dvmlim96@kribb.re.kr
- 8Department of Internal Medicine and Cardiovascular Center, Chosun University Hospital, University of Chosun College of Medicine, Gwangju 61453, Korea.
- KMID: 2452876
- DOI: http://doi.org/10.12729/jbtr.2019.20.2.044
Abstract
- Acute myocardial infarction (AMI) is considered the major cause of mortality in the world. Tremendous animal studies are performed to develop novel therapeutics, and this study aimed to induce porcine myocardial infarction model by using polyethylene terephthalate (PET). Coronary guidewire was placed in left anterior descending artery (LAD). The balloon angioplasty catheter was inserted at the back of the PET. The balloon catheter was carefully pushed forward, until the balloon marker was located in mid-LAD. Coronary angiography was performed pre- and post-occlusion at 28 days by C-arm. Histologic analysis of heart tissue was performed 28 days after inducing AMI. Thirty three pigs were anesthetized and underwent percutaneous coronary catheterization. All pigs were successfully embolized in mid-LAD by PET. Fifteen pigs died due to ventricular fibrillation during post-anesthetic recovery time, and overall experiment mortality was 45.5%. In 2,3,5-triphenyl tetrazolium chloride staining, gross finding of the ischemic heart lesion showed firm and white area of infarction associated with the apex and left ventricular posterior wall. Infarct on H&E-stained sections demonstrated a region without myocytes and rich with cardiomyocyte with atypical nuclei. Successful induction of AMI by using PET may provide the pathophysiological information of ischemic heart disease and improvement of therapy development for AMI.
Keyword
MeSH Terms
-
Angioplasty, Balloon
Animals
Arteries
Cardiovascular Diseases
Catheterization
Catheters
Coronary Angiography
Heart
Infarction
Mortality
Muscle Cells
Myocardial Infarction*
Myocardial Ischemia
Myocytes, Cardiac
Polyethylene Terephthalates*
Polyethylene*
Swine
Ventricular Fibrillation
Polyethylene
Polyethylene Terephthalates
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