Int J Oral Biol.  2019 Jun;44(2):55-61. 10.11620/IJOB.2019.44.2.55.

Effect of Garcinia mangostana L. and propolis extracts on the inhibition of inflammation and alveolar bone loss in ligature-induced periodontitis in rats

• Sung SJ
• Kang KM
• Lee KH
• Yoo SY
• Kook JK
• Lee DS
• Yu SJ

• KMID: 2452748
• DOI: http://doi.org/10.11620/IJOB.2019.44.2.55

Abstract

The purpose of this study was to evaluate the effect of mangosteen extract complex (MEC; Garcinia mangostana L. and propolis extracts) on the inhibition of inflammation and prevention of alveolar bone loss using a ligature-induced periodontitis model. Rat molars were ligatured with silk, and 1 µg/mL of lipopolysaccharide of Porphyromonas gingivalis was injected into the buccal and palatal gingivae of the teeth with or without treatment with the MEC. Changes in the expression levels of prostaglandin Eâ‚‚ (PGEâ‚‚), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), matrix metalloproteinase-8 (MMP-8), cyclooxygenase (COX)-1, and COX-2 in gingival tissues were evaluated using enzyme-linked immunosorbent assays. Alveolar bone loss around the ligated molars was examined using micro-computed tomography. The expression levels of PGEâ‚‚, IL-8, iNOS, MMP-8, COX-1, and COX-2 in gingival tissues were significantly reduced in the group treated with a mixture of 16 µg of mangosteen extract powder and 544 µg of propolis extract powder (ligation [Lig] + lipopolysaccharide extracted from P. gingivalis KCOM 2804 [L] + MEC 1:34). Additionally, alveolar bone loss was significantly reduced in the Lig + L + MEC 1:34 group compared with that in other groups. These results indicate that the MEC could be useful in preventing and treating periodontitis.

Keyword

Mangosteen extract complex; Anti-inflammatory; Alveolar bone loss

MeSH Terms

Alveolar Bone Loss*
Animals
Enzyme-Linked Immunosorbent Assay
Garcinia mangostana*
Garcinia*
Gingiva
Inflammation*
Interleukin-8
Matrix Metalloproteinase 8
Molar
Nitric Oxide Synthase Type II
Periodontitis*
Porphyromonas gingivalis
Propolis*
Prostaglandin-Endoperoxide Synthases
Rats*
Silk
Tooth
Interleukin-8
Matrix Metalloproteinase 8
Nitric Oxide Synthase Type II
Propolis
Prostaglandin-Endoperoxide Synthases
Silk