Obstet Gynecol Sci.  2019 Jul;62(4):264-272. 10.5468/ogs.2019.62.4.264.

Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus

• Yayla AE
• Kadioglu B
• Aydin A
• Aktas O

• KMID: 2451656
• DOI: http://doi.org/10.5468/ogs.2019.62.4.264

Abstract

OBJECTIVE
Human papillomaviruses (HPVs) are among the agents responsible for infection and cancer of the skin and mucous membranes in the human body. The aim of this study was to investigate the frequency and type distribution of HPVs in married female patients with gynecological complaints, who had visited the Maternity Hospital in Erzurum, Turkey.
METHODS
In this study, 263 cervical swab samples were taken from married women using the Pap smear method and were investigated for positive reactivity against HPV. The L1 gene region of HPV was investigated using molecular methods. For this purpose, polymerase chain reaction (PCR) assays and sequence analysis of positive samples were performed. Phylogenetic analyses were performed using a bioinformatics approach after sequencing.
RESULTS
HPV-DNA was detected in 17 (6.5%) samples. Highest positive reactivity to HPV-DNA was found in the 35-44 age group at 9.2%. Fourteen out of seventeen positive samples were included in the phylogenetic analysis. All isolates clustered in the Alphapapillomavirus genus. Six samples were found to be HPV 70 positive, four were HPV 16 positive, and the rest were HPV 54, 72, 81, and 114 positive. When genotyping data were evaluated according to the risk group, we found that 28.6% of the 14 samples were found to be high risk-HPV, and 71.4% were low risk-HPV.
CONCLUSIONS
As per our knowledge, this is the first report on the phylogenetic analysis of HPV genotypes isolated from women in Turkey. The prevalence of low- and-high risk HPV was determined in married women in Erzurum, and these results contribute to the epidemiological data on the distribution of HPV types for this region.

Keyword

Papillomavirus infections; Cervical smears; Polymerase chain reaction; Turkey

MeSH Terms

Alphapapillomavirus
Computational Biology
Female
Genotype
Hospitals, Maternity
Human Body
Human papillomavirus 16
Humans*
Methods
Mucous Membrane
Papanicolaou Test
Papillomavirus Infections
Polymerase Chain Reaction
Prevalence*
Sequence Analysis
Skin Neoplasms
Turkey
Vaginal Smears

Figure

• 

  Fig. 1 Phylogenetic analysis of the L1 gene region in human papillomaviruses (HPV)-DNA positive specimens. HPVs classified in five different genera are indicated in the phylogenetic tree. All of our study samples in which the Alpha genus was included are shown with rounded black dots. Other examples mentioned in the phylogenetic tree were obtained from the National Center for Biotechnology Information (NCBI) GenBank and are shown by the access numbers.

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