J Korean Acad Pediatr Dent.  2019 May;46(2):135-138. 10.5933/JKAPD.2019.46.2.135.

Susceptibility of Mutans streptococci in the Planktonic and Biofilm State to Erythrosine

• Gong J
• Seo H
• Lee S
• Park H
• Lee J

• KMID: 2449973
• DOI: http://doi.org/10.5933/JKAPD.2019.46.2.135

Abstract

The aim of this study was to investigate the susceptibility of Mutans streptococci in both planktonic and biofilm states to erythrosine. S. mutans was cultured in brain-heart infusion (BHI) broth. Erythrosine was diluted in BHI broth and prepared at a concentration range of 0.02 - 10000 µg/L. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured using the microdilution method. After forming biofilms on 96-well plates, the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were measured. S. mutans was susceptible to erythrosine in both planktonic and biofilm states. MIC and MBC values were both 19.5 µg/L for the planktonic state, while MBIC and MBEC values were 313 µg/L and 2500 µg/L, respectively, for the biofilm state. Erythrosine (19.5 µg/L) exhibited a bactericidal effect on S. mutans (killing 99.9%) in the planktonic state. For biofilms, erythrosine inhibited biofilm growth and eradicated 99.9% of biofilm bacteria at higher concentrations than MIC and MBC. These MBIC and MBEC concentrations are much lower than known noxious doses, and the MIC, MBC, and MBIC values were even lower than clinical concentrations.

Keyword

Erythrosine; Mutans streptococci; Biofilm; Susceptibility

MeSH Terms

Bacteria
Biofilms*
Erythrosine*
Methods
Microbial Sensitivity Tests
Plankton*
Erythrosine