Korean J Parasitol.  2019 Apr;57(2):83-92. 10.3347/kjp.2019.57.2.83.

Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells

• Sun PR
• Gao FF
• Choi HG
• Zhou W
• Yuk JM
• Kwon J
• Lee YH
• Cha GH

• KMID: 2448139
• DOI: http://doi.org/10.3347/kjp.2019.57.2.83

Abstract

Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.

Keyword

T. gondii; reactive oxygen species; diphenyleneiodonium; N-acetyl-L-cysteine; ARPE-19 cell

MeSH Terms

Acetylcysteine
Ascorbic Acid
Humans
Mitochondria
Parasites
Reactive Oxygen Species
Retinaldehyde
Superoxides
Toxoplasma*
Toxoplasmosis
Acetylcysteine
Ascorbic Acid
Reactive Oxygen Species
Retinaldehyde
Superoxides