Nat Prod Sci.  2019 Mar;25(1):23-27. 10.20307/nps.2019.25.1.23.

Cytotoxic Lactones from the Pericarps of Litsea japonica

• Ngo QM
• Cao TQ
• Woo MH
• Min BS

• KMID: 2443099
• DOI: http://doi.org/10.20307/nps.2019.25.1.23

Abstract

From the pericarps of Litsea japonica (Thunb.) Jussieu, eighteen butanolide derivatives (1 - 18) were evaluated for their cytotoxic activity against HeLa, HL-60, and MCF-7 cells. Compounds 1 - 9 with 2-alkylidene-3-hydroxy-4-methylbutanolides structure exhibited cytotoxic activities against cancer-cell lines. Among them, compound 8 (litsenolide Dâ‚‚) exhibited the most potent cytotoxicity against the tested cell lines, including HeLa, HL-60, and MCF-7, with ICâ‚…â‚€ values of 17.6 ± 1.3, 4.2 ± 0.2, and 12.8 ± 0.0 µM, respectively. Compound 8 induced apoptosis in a dose-dependent manner. Annexin V/Propidium Iodide (PI) double staining confirmed that 8 effectively induced apoptosis in MCF-7 cells. To the best of our knowledge, we have reported cytotoxic activity of butanolides from L. japonica against these cancer-cell lines for the first time.

Keyword

Litsea japonica; Lauraceae; butanolide; lactone; cytotoxic activity; apoptosis

MeSH Terms

Apoptosis
Cell Line
Lactones*
Lauraceae
Litsea*
MCF-7 Cells
Lactones

Figure

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  Fig. 1. The structures of compounds 1–18 from the pericarps of Litsea japonica.

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  Fig. 2. SAR summary of butanonolide derivatives.

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  Fig. 3. Flow cytometric analysis of compound 8-induced apoptosis in MCF-7 cell using annexin-V-FLUOS/PI. Cells (1 × 106 cells) were incubated with indicated concentration of 8 for 24 h and stained with annexin-V-FLUOS/PI to analysis apoptosis and necrotic cell populations. Cells in the lower right quadrant represented apoptosis and upper right quadrant. Data are representative of one of three similar experiments.

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