Osong Public Health Res Perspect.
2019 Feb;10(1):25-31. 10.24171/j.phrp.2019.10.1.06.
Analysis of Resistance to Macrolide–Lincosamide–Streptogramin B Among mecA-Positive Staphylococcus Aureus Isolates
- Affiliations
-
- 1Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
- 2Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
- 3Department of Pathology, Shafa Hospital, Qazvin University of Medical Sciences, Qazvin, Iran.
- 4Department of Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
- 5Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
- 6Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
- 7Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
- 8Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran. ramazan69@yahoo.com
- KMID: 2441510
- DOI: http://doi.org/10.24171/j.phrp.2019.10.1.06
Abstract
OBJECTIVES
Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLSB) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLSB resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples.
METHODS
A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLSB-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates.
RESULTS
The frequency of constitutive resistance MLSB, inducible resistance MLSB and MSB resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLSB phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLSB phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively.
CONCLUSION
Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Prevalence of Inducible Macrolide-Lincosamide-Streptogramin B (MLS(B)) Resistance in Erythromycin-Resistant Staphylococci
- Distributions of Macrolide-Lincosamide-Streptogramin B Resistance Phenotypes in Clinical Isolates of Staphylococi
- The Methicillin-Resistance Rate of Staphylococcus aureus Isolatd from Anterior Nares of Healthy Adults in the Community
- Distributions of Macrolide-Lincosamide-Streptogramin (MLS) Resistance Types in beta-hemolytic Streptococci
- Acquisition of methicillin resistance and progression of multiantibiotic resistance in methicillin-resistant Staphylococcus aureus
