Korean J Dermatol.
2019 Feb;57(2):59-65.
Gene Expression in Cultured Keloid Fibroblasts
- Affiliations
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- 1Department of Dermatology, Wonkwang University School of Medicine, Iksan, Korea. derma@chol.com
- 2Beautiful Dermatology Clinic, Gochang, Korea.
- 3Department of Pathology, Wonkwang University School of Medicine, Iksan, Korea.
Abstract
- BACKGROUND
Keloids are the result of an overgrowth of dense fibrous tissue. The mechanisms involved in keloid formation remain unknown.
OBJECTIVE
To understand the mechanism of keloid formation, differences in gene expression in keloids and normal skin were compared. Gene expression profiles of keloids and normal skin were analyzed.
METHODS
From microarray analysis, eight genes whose expression profiles showed down-regulation or up-regulation in keloid samples were selected and the expression of these genes were confirmed by real-time polymerase chain reaction to further shed light on the mechanism of keloid formation.
RESULTS
The expression levels of FBXO32, NTF3, MEGF6, and ITGA8 were significantly increased in cells isolated from keloid tissue, whereas the expression levels of PTGS2, PITX1, MALL, and AMFR were significantly decreased in keloids compared to normal skin.
CONCLUSION
These differently expressed genes in keloids and normal skin may play an important role in keloid formation.
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