Korean J Vet Res.  2018 Dec;58(4):211-217. 10.14405/kjvr.2018.58.4.211.

Differentiation potential of canine mesenchymal stem cells on hydrogel scaffold-based three-dimensional environment

• Gu NY
• Park MJ
• Lee J
• Byeon JS
• Jeong DU
• Cho IS
• Cha SH

• KMID: 2437696
• DOI: http://doi.org/10.14405/kjvr.2018.58.4.211

Abstract

Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-β1 and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3DTN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.

Keyword

chondrogenesis; extracellular matrix; hydrogels; mesenchymal stem cells; osteogenesis

MeSH Terms

Bone Regeneration
Cell Proliferation
Cell- and Tissue-Based Therapy
Chondrogenesis
Collagen Type II
Extracellular Matrix
Hydrogel*
Hydrogels
Mesenchymal Stromal Cells*
Osteocalcin
Osteogenesis
Polymers
Proliferating Cell Nuclear Antigen
Tissue Engineering
Water
Collagen Type II
Hydrogel
Hydrogels
Osteocalcin
Polymers
Proliferating Cell Nuclear Antigen
Water