Cancer Res Treat.  2019 Jan;51(1):337-344. 10.4143/crt.2018.157.

Founder Mutations for Early Onset Melanoma as Revealed by Whole Exome Sequencing Suggests That This is Not Associated with the Increasing Incidence of Melanoma in Poland

• DÄ™bniak T
• Scott RJ
• Lea RA
• Górski B
• Masojć B
• Cybulski C
• Kram A
• Maleszka R
• Gromowski
• Paszkowska-Szczur K
• Kashyap A
• Lener MR
• MaliÅ„ska
• Rogoża E
• Murawa D
• Rudnicka H
• DeptuÅ‚a J
• LubiÅ„ski

• KMID: 2437624
• DOI: http://doi.org/10.4143/crt.2018.157

Abstract

PURPOSE
Germline mutations within melanoma susceptibility genes are present only in minority of melanoma patients and it is expected that additional genes will be discovered with next generation sequence technology and whole-exome sequencing (WES).
MATERIALS AND METHODS
Herein we performed WES on a cohort of 96 unrelated Polish patients with melanoma diagnosed under the age of 40 years who all screened negative for the presence of CDKN2A variants. A replication study using a set of 1,200 melanoma patient DNA samples and similarly large series of healthy controls was undertaken.
RESULTS
We selected 21 potentially deleterious variants in 20 genes (VRK1, MYCT1, DNAH14, CASC3, MS4A12, PRC1, WWOX, CARD6, EXO5, CASC3, CASP8AP2, STK33, SAMD11, CNDP2, CPNE1, EFCAB6, CABLES1, LEKR1, NUDT17, and RRP15), which were identified by WES and confirmed by Sanger sequencing for an association study. Evaluation of the allele distribution among carriers and their relatives in available family trios revealed that these variants were unlikely to account for many familial cases of melanoma. Replication study revealed no statistically significant differences between cases and controls.
CONCLUSION
Although most of the changes seemed to be neutral we could not exclude an association between variants in VRK1, CREB3L3, EXO5, and STK33 with melanoma risk.

Keyword

Whole exome sequencing; Melanoma; Frameshift mutation

MeSH Terms

Alleles
Cohort Studies
DNA
Exome*
Frameshift Mutation
Germ-Line Mutation
Humans
Incidence*
Melanoma*
Poland*
DNA

Reference

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