Int J Oral Biol.  2018 Dec;43(4):201-207. 10.11620/IJOB.2018.43.4.201.

Antibody-based Screening of Porphyromonas gingivalis Proteins Specifically Produced in Patients with Chronic Periodontitis

• Kim HJ
• Lee SW

• KMID: 2437337
• DOI: http://doi.org/10.11620/IJOB.2018.43.4.201

Abstract

Porphyromonas gingivalis is among the major etiological pathogens of chronic periodontitis. The virulence mechanisms of P. gingivalis is yet to be identified as its activity is largely unknown in actual disease process. The purpose of this study is to identify antigens of P. gingivalis expressed only in patients with chronic periodontitis using a unique immunoscreening technique. Change Mediated Antigen Technology (CMAT), an antibody-based screening technique, was used to identify virulence-associated proteins of P. gingivalis that are expressed only during infection stage in patients having chronic periodontitis. Out of 13,000 recombinant clones screened, 22 tested positive for reproducible reactivity with rabbit hyperimmune anti-sera prepared against dental plaque samples acquired from periodontitis patients. The DNA sequences of these 18 genes were determined. CMAT-identified protein antigens of P. gingivalis included proteins involved in energy metabolism and biosynthesis, heme and iron binding, drug resistance, specific enzyme activities, and unknown functions. Further analysis of these genes could result in a novel insight into the virulence mechanisms of P. gingivalis.

Keyword

In vivo; Virulence factors; Antigens; Chronic periodontitis; Porphyromonas gingivalis

MeSH Terms

Base Sequence
Chronic Periodontitis*
Clone Cells
Dental Plaque
Drug Resistance
Energy Metabolism
Heme
Humans
Iron
Mass Screening*
Periodontitis
Porphyromonas gingivalis*
Porphyromonas*
Virulence
Virulence Factors
Heme
Iron
Virulence Factors