Korean J Blood Transfus.  2018 Dec;29(3):291-300. 10.17945/kjbt.2018.29.3.291.

Freezing and Washing of Red Blood Cells Using Haemonetics ACP 215

• Youn KW
• Choi KY
• Lee SA
• Min HK
• Kim J

• KMID: 2435623
• DOI: http://doi.org/10.17945/kjbt.2018.29.3.291

Abstract

BACKGROUND
The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim of this study was to assess glycerolization and deglycerolization processing for RBCs using a high glycerol method in the automated, closed system provided by Haemonetics ACP 215.
METHODS
Thirty-five packed RBCs were glycerolized using the ACP 215 to a final concentration of 40% (wt/vol). The units were either frozen as such (n=30) or excess glycerol was removed (n=5) before freezing. After storage at −80℃, the units were thawed, deglycerolized and resuspended in SAG-M. The frozen-thawed RBCs were stored at 4℃, and analyzed for their stability and in vitro quality.
RESULTS
No prefreeze excess glycerol removal units showed significantly less potassium leakage during post-thaw storage compared to the prefreeze excess glycerol removal units. All measurements of the stability and in vitro quality of thawed RBCs prepared from frozen RBCs without the prefreeze removal of excess glycerol during post-thaw storage at 4℃ for 7 days were acceptable to the American Blood Bank Association's standards and European standards.
CONCLUSION
RBCs frozen without prefreeze removal of excess glycerol and the ACP 215 simplifies cryopreservation procedure and increases the stability of frozen-thawed RBCs. This increases the practical applicability of cryopreserved RBCs in blood transfusion practice.

Keyword

Red blood cells; Cryopreservation; Glycerolization; Deglycerolization

MeSH Terms

Blood Banks
Blood Transfusion
Cryopreservation
Erythrocytes*
Freezing*
Glycerol
In Vitro Techniques
Methods
Potassium
Glycerol
Potassium