Peripheral blood stem cell collections using a Haemonetics MCS3p

Lee, Woo In; Suh, Jin Tae

Abstract

BACKGROUND
Reconstitution of hematopoiesis by means of peripheral blood stem cells (PBSC) has been widely used in patients with hematologic malignancies or solid tumors. We analyzed our experiences of PBSC collections with a MCS3p (Haemonetics Corp., Braintree, MA,USA) and investigated factors that may affect the collection of PBSC. METHOD: The forty-seven harvests in 11 cancer patients (median age 48, 4 males : 7 females) were performed during marrow recovery after administration of anticancerous regimens and and hematopoietic growth factors.
RESULTS
Median number of WBC & mononuclear cells (MNCs) in preapheresis was 4,700/microliter and 1,472/microliter, retrospectively. A total of 11.2 x 109 (range 5.4~17.0) MNCs were collected from 7.2l (5.5~8.9) blood processed per procedure. The mean percentage of CD34 positive cells in the product was 1.9% (0.9~2.9). Collection efficiency was 58.1%. While age of patients or total processed blood volume did not affect for total number of MNC collected, the number of WBCs including MNCs on the day of harvest was an importanr factor for higher yield of MNCs (P<0.05).
CONCLUSION
We concluded that Haemonetics MCS3p is a fully automated blood cell separator with good collection efficiency for hematopoietic progenitor cells.