Clin Hypertens.  2018 ;24(1):5. 10.1186/s40885-018-0090-8.

Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens

• Youn JC
• Kim JY
• Jung MK
• Yu HT
• Park SH
• Kim IC
• Lee SK
• Choi SW
• Han S
• Ryu KH
• Park S
• Shin EC

• KMID: 2434259
• DOI: http://doi.org/10.1186/s40885-018-0090-8

Abstract

BACKGROUND
Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension.
METHODS
To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays.
RESULTS
In patients with hypertension, senescent CD8+ T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8+ T-cell responses as measured by ICS. CMV-specific CD8+ T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes
CONCLUSIONS
CMV pp65-specific CD8+ T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.

Keyword

Cytomegalovirus; T cell; Arterial stiffness; Enzyme-linked immunospot (ELISPOT) assay; Intracellular cytokine staining (ICS)

MeSH Terms

Cytomegalovirus
Enzyme-Linked Immunospot Assay
Humans
Hypertension*
Methods*
Pulse Wave Analysis
T-Lymphocytes*
Vascular Stiffness