Nutr Res Pract.  2016 Oct;10(5):487-493. 10.4162/nrp.2016.10.5.487.

Korean Curcuma longa L. induces lipolysis and regulates leptin in adipocyte cells and rats

Affiliations
  • 1Department of Food and Nutrition, International University of Korea, 965, Dongbu-ro, Munsan-eup, Jinju, Gyeongnam 52833, Korea. jhappychoi@naver.com

Abstract

BACKGROUND/OBJECTIVES
Turmeric (Curcuma longa L.) has been reported to have many biological functions including anti-obesity. Leptin, peptide hormone produced by adipocytes and its concentration is increased in proportion to the amount of the adipocytes. In the present study, we examined the effects of Korean turmeric on the regulation of adiposity and leptin levels in 3T3-L1 adipocytes and rats fed a high-fat and high-cholesterol diet.
MATERIALS/METHODS
Leptin secretion, free fatty acid and glycerol contents in 3T3-L1 adipocytes were measured after incubation of cells with turmeric for 24 hours. Rats were divided into four experimental groups: a normal diet group (N), a high-fat and high-cholesterol diet group (HF), a high-fat and high-cholesterol diet group supplemented with 2.5% turmeric extracts (TPA group) and a high-fat and high-cholesterol diet group supplemented with 5% turmeric extracts (TPB group). Serum samples were used for the measurement of leptin concentration.
RESULTS
Contents of free fatty acid and glycerol showed concentration dependent increase in response to turmeric extracts. Effects of turmeric extracts on reduction of lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Treatment with turmeric extracts resulted in increased expression levels of adipose triglyceride lipase and hormone-sensitive lipase mRNA. The concentration of leptin from 3T3-L1 adipocytes was significantly decreased by turmeric. Proportional abdominal and epididymal fats weights of the turmeric 5% supplemented group, TPB has significantly decreased compared to the HF group. The serum levels of leptin in the TPA and TPB groups were significantly lower than those of the HF group.
CONCLUSIONS
Based on these results, we suggested that Korean turmeric may contribute to the decreasing of body fat and regulating leptin secretion.

Keyword

Turmeric; curcumin; leptin; free fatty acid; glycerol

MeSH Terms

3T3-L1 Cells
Adipocytes*
Adipose Tissue
Adiposity
Animals
Curcuma*
Curcumin
Diet
Fats
Glycerol
Leptin*
Lipase
Lipolysis*
Rats*
RNA, Messenger
Sterol Esterase
Weights and Measures
Curcumin
Fats
Glycerol
Leptin
Lipase
RNA, Messenger
Sterol Esterase

Figure

  • Fig. 1 Effects of turmeric extracts on viability of differentiating 3T3-L1 adipocytes. MTT colorimetric assay testing cell viability was performed after 24 hours of treatment with indicated concentration of turmeric. All values are presented as means ± SE. (n≥3). *Statistical significance determined by Tukey's test at P < 0.05.

  • Fig. 2 Effects of turmeric extracts on free fatty acid (FFA) (A) and glycerol (B) of differentiating in 3T3-L1 adipocytes. FFA and glycerol assay was performed after 24 hours of treatment with indicated concentrations of turmeric extracts. Control indicates a sample stimulated only with medium. All values are presented as means ± SE. (n≥3). *Statistical significance determined by Tukey's test at P < 0.05. vs control.

  • Fig. 3 Turmeric extracts inhibit lipid accumulation in 3T3-L1 adipocytes. Adipocytes were incubated with three concentrations (2.5, 5 and 10 µg/ml) of turmeric extracts and lipid accumulation was determined by Oil Red O staining and photographed. Lipid droplet formation in mature adipocytes (20X).

  • Fig. 4 Expression of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA from 3T3-L1 adipocytes stimulated by turmeric extracts. The effect of turmeric extracts on ATGL and HSL mRNA levels were investigated in 3T3-L1 adipocytes after 24 hours of treatment by RT-PCR analysis (A). Representative 2% agarose gel electrophoresis of PCR products stained with ethidium bromide. The right graphs show the mean band densitometry of ATGL or HSL/GAPDH relative expression.

  • Fig. 5 Secretion of leptin from 3T3-L1 adipocytes in response to turmeric extracts. Leptin assay in 3T3-L1 adipocytes was performed after 24 hours of treatment with indicated concentrations of turmeric extracts. Control indicates a sample stimulated only with medium. All values are presented as means ± SE. (n≥3). *Statistical significance determined by Tukey's test at P < 0.05. vs control.

  • Fig. 6 Effects of turmeric extracts on serum leptin of rats fed high-fat and high-cholesterol diets. The secretion of leptin from serum of rats fed normal diet (N), high-fat and high-cholesterol diet (HF) and high-fat and high-cholesterol diet with turmeric extracts 2.5% (TPA) or 5% (TPB) diets for 4 weeks. All values are the means ± SE (n = 10). Those with different superscript letters are significantly different at P < 0.05 by Tukey's test.


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