Ann Dermatol.  2019 Feb;31(1):97-100. 10.5021/ad.2019.31.1.97.

Poly-L-Lactic Acid Increases Collagen Gene Expression and Synthesis in Cultured Dermal Fibroblast (Hs68) Through the p38 MAPK Pathway

• Kim SA
• Kim HS
• Jung JW
• Suh SI
• Ryoo YW

• KMID: 2430812
• DOI: http://doi.org/10.5021/ad.2019.31.1.97

Abstract

No abstract available.

MeSH Terms

Collagen*
Fibroblasts*
Gene Expression*
p38 Mitogen-Activated Protein Kinases*
Collagen
p38 Mitogen-Activated Protein Kinases

Figure

• 

  Fig. 1 Effect of PLLA on collagen synthesis. (A) Human fibroblast cell line (Hs68) was treated with PLLA at 0.1% concentration for 24 and 48 hours. Type I collagen expression was determined using real time RT-PCR. The relative levels of mRNA were normalized against GAPDH levels from the same cDNA preparation. Data represent the mean±SD of the results in three independent experiments. *p<0.05. (B) The levels of type I procollagen protein were determined using ELISA for 24 and 48 hours. Data represent the mean±SD of the results in three independent experiments. **p<0.05. (C) The levels of type I collagen protein were determined using the Western blot analysis for 24 and 48 hours. (D) The relative levels of type I collagen protein were normalized against beta-actin levels from the same protein preparation. Data represent the mean±SD of the results in three independent experiments. **p<0.01, PLLA: poly-L-lactic acid, RT-PCR: reverse transcription-polymerase chain reaction, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, SD: standard deviation, ELISA: enzyme-linked immunosorbent assay.

• 

  Fig. 2 (A) Activation of p38, JNK, and Akt is critical for PLLA-induced collagen I gene expression. Human dermal fibroblasts (HS68) were treated with PLLA (0.1%) for 24 and 48 hours. At each time, cell lysates were prepared and used for type I collagen, p-p38 MAPK, p38 MAPK, p-JNK, JNK, p-Akt, Akt, p-ERK, and ERK Western blot analyses with respective antibodies. (B) Data represent the mean±SD of the results in three independent experiments. *p<0.05. (C) Effects of MAPK inhibitors on PLLA-induced type I collagen production. Hs68 cells were pretreated with the indicated concentration of SB203580 (p38/Akt inhibitor) and SP600125 (JNK inhibitor) for 30 minutes and treated with 0.1% PLLA for an additional 48 hours. Cell lysates were prepared and used for p-p38 MAPK, p-JNK, p-Akt, p-ERK, and Western blot analyses with respective antibodies. (D) Data represent the mean±SD of the results in three independent experiments. *p<0.05, **p<0.01, ***p<0.001. PLLA: poly-L-lactic acid, p-: phosphorylated, SD: standard deviation, MAPK: mitogen-activated protein kinase, SB: SB203580 (p38/Akt inhibitor), SP: SP6001.

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